CPL Spectrophotometer

The CPL-300 uses the original 180° fluorescence collection approach proposed by Steinberg. The standard ozone-free 150W Xe lamp can be user-replaced with an Hg/Xe source. The instrument’s double prism excitation and emission monochromator delivers very low stray light and no spurious linear polarization effects caused by diffraction gratings.

Circularly polarized luminescence (CPL) offers complementary information to other chiroptical techniques such as Optical Rotatory Dispersion (ORD), Circular Dichroism (CD) and Vibrational Circular Dichroism (VCD).

CPL 300
CPL-300 Circularly Polarized Luminescence Spectrophotometer
Circularly Polarized Luminescence Spectrophotomete

Principles of Circularly Polarized Luminescence


Optical system


System Features

Measurement of solid-state sample

Chiral properties of molecules in both liquid and solid states can differ greatly. Many of the devices used in today’s advanced technological fields of research are based on circularly polarized light-emitting molecules. CPL is an ideal technique for use with these solid-state samples.

The CPL-300’s unique 180° linear optical design provides pure, unpolarized excitation light allowing the elimination of artifacts due to fluorescence anisotropy. Samples such as KBr pellets and PMMA films are easily measured providing highly reliable CPL spectra). CPL measurements on samples with known macroscopic anisotropy such as single crystals are routinely reported in literature using a dedicated CPL spectrometer.

In addition, measurements on solid-state samples are being reported today using the JASCO CPL-300 Spectrometer.


System Features

A CPL signal

A CPL signal is the intensity difference between left and right circularly polarized light emitted by a chiral molecule which is excited with unpolarized light. The circularly polarized luminescence spectrophotometer supplements the information obtained from other chiroptical techniques such as electronic or vibrational circular dichroism.

Circular dichroism (CD) spectroscopy

Circular dichroism (CD) spectroscopy is widely used in the study of optically active substances. CD spectroscopy provides information about the structure of optically active substances in the electronic ground state, however, CPL spectroscopy provides information about excited states. The two techniques are therefore complimentary.


Materials that exhibit circularly polarized luminescence are being actively investigated in applications such as liquid crystal display backlights, 3D displays, holographic displays, light sources for controlling plant growth, and security systems for optical communications and printing.


It is therefore important to identify molecules that emit one-handed circularly polarized light with high quantum yield. CPL spectra contain important information such as the stable structure of excited molecules, and the behavior of these molecules in chemical reactions that involve an intermediate excited state. However, fluorescence emission is generally weak and CPL signals are even weaker, making it difficult to detect. The CPL-300 offers high sensitivity for detection of these weak signals over a wide spectral range.

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