SFE-4000 System

CO2 delivery pumps

The CO2 pumps (with flow rate up to 120 ml/min) include Peltier cooling (with pump-head temperature monitoring) to control the density of the mobile phase for accurate CO2 flow.

Automatic, shut-off valves close the CO2 inlet and outlet (and co-solvent pump) to isolate the pumps for quick and simple priming when flow is not pumping.

Preparative CO2 pump – PU-4388
Preparative CO2 pump – PU-4388
Back Pressure Regulator – BP-4340
Back Pressure Regulator – BP-4340

Back pressure regulator

The patented back pressure regulator has unmatched pressure regulation precision and accuracy with an extremely low noise baseline and excellent retention time reproducibility.

Gas-liquid separator

The micro-cyclone separators provide simple gas-liquid separation in the fraction vial typically yielding better than 95 % recoveries.

Micro-cyclone separator
Micro-cyclone separator

SFE Vessel Ovens

The SFE-4000 Series vessel ovens have been designed to provide excellent utility for a wide range of different user requirements.
All SFE vessel ovens have built-in electronic cooling for controlling vessel temperature at sub-ambient temperatures.


For analytical and larger preparative vessels, select either of the two high-capacity CO-4060 or CO-4065 vessel ovens.

Extraction Vessels

There are a variety of extraction vessels with designs specific for solids and liquids with a wide range of volumes.

Extraction Vessel
Extraction Vessel
UV-Vis Detector – UV-4070
UV-Vis Detector – UV-4070

SFE Detectors lineup

With JASCO’s long history of spectrometer development, you will find that each detector is well designed; these next generation detectors employ the latest optical and electronic technologies for high sensitivity, excellent stability and easy maintenance.

UV-Visible Detectors

The UV-4070 (190 – 900 nm) and UV-4075 (190 – 600 nm) UV-visible detectors both use an advanced Czerny-Turner monochromator with dual wavelength and scanning functions.

Baseline change due to refractive index is minimized by the flow cell design, and fluctuation from changes in environmental and lamp temperature are reduced with a regulated lamp housing and temperature controlled cell.

PDA Detectors

The SFE-4000 Series includes three PDA detectors. The MD-4017 is a simple replacement for a single wavelength detector, the MD-4015 is the workhorse for UV-Visible detection in the range 200 to 650 nm and the MD-4010 has a wide wavelength range.

When used with ChromNAV 2.0 CDS the PDA detector offers multi-functional analysis with a variety of functions such as spectral analysis, library search for peak identification, peak purity and multi-wavelength quantitation.

ChromNAV 2.0 Software

The latest version of ChromNAV 2.0 software is a simple to use, and powerful Chromatography Data System (CDS). The highly configurable graphical-user-interface (GUI) can be quickly set up to display only the functions necessary for your use.

Users can quickly learn the operation and explore the extensive functionality for instrument control and data processing.


ChromNAV 2.0 is a universal CDS which can be used with any type of analytical separation, including: HPLC, UHPLC, GPC, SFC/SFE and preparative HPLC & SFC/SFE.

Check out the complete suite of option applications below.

  • ChromNAV CFR – Fully 11 CFR part II compliant version of ChromNAV, with electronic signature and complete audit trail.
  • ChromNAV-Fraction Controller – for controlling fraction collection either by open bed fraction collector or fixed fraction valve, using timed events and threshold triggering.
ChromNAV 2.0 HPLC Software
ChromNAV 2.0 HPLC Software

ChromNav 2.0 Standard Features

All raw data is protected and saved, which can then be analyzed and re-analyzed, reported and saved with both the raw data and any data processing from the user’s analysis.

A comprehensive audit trail records the acquisition method along with a history of instrument performance indicators in each sample data file, which provides the user a snap-shot of the condition of the system during the run and can warn about requirements for impending maintenance.