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V-750 UV-Visible/NIR Spectrophotometer

The V-750 is a high resolution UV-visible double-beam spectrophotometer with single monochromator, variable spectral bandwidth and a photomultiplier tube (PMT) detector

System Description

The optical design employs ‘true double beam optics’ with a chopper providing timed sample, reference and dark current measurements. A high sensitivity PMT (photomultiplier tube) provides accurate and reproducible measurements of both low (and high) concentration samples. By controlling the high voltage applied to the PMT, the dynode feedback circuit allows a wider dynamic range. It also provides excellent sensitivity for diffuse reflectance or transmittance of solid and liquid samples using an integrating sphere. The spectral bandwidth can be set as narrow as 0.1 nm for high resolution measurements, such as gas and vapor phase spectroscopy. Special low stray light slit settings which optimize the light image on the monochromator provide increased linearity of up to 5 absorbance units.

With a comprehensive range of sampling accessories, the V-750 is well suited to the measurement of samples in the UV and visible regions, with temperature control for biological samples and a number of transmittance and reflectance accessories for materials analysis.

Operating Systems

Two user interfaces are available. The Spectra Manager™ II software, the latest version of JASCO's innovative cross-platform spectroscopy software, is now compatible with Windows™ 7 Pro (32- and 64-bit configurations) and the Windows™ 8.1 operating systems.

For simple and routine operation, the intelligent remote module (iRM) has been completely redesigned with a new look and feel, with a color LCD touch screen. Data can also be downloaded to a USB memory stick for portability or further PC data processing.

The V-700 Series has a growing list of software applications for both Spectra Manager II and for the iRM. If you have an application which you don’t see listed, please let us know as we may already have it or we can prepare an application designed specifically for your requirements.

Both of these interfaces allow full system control and advanced data processing. Spectra Manager CFR is the 21 CFR part 11 compliant version of software and is available as an option.

System Features

IQ Accessory and IQ Start

The IQ Accessory function provides automated accessory recognition with identification of the accessory within the method. The spectral data files include the accessory information and serial number while designated instrument parameters can be configured and loaded for optimum sample measurements. IQ Start together with the Start Button is used to immediately start a sample method for immediate access when making routine measurements.

Excellent Optical Performance

The most recent advances in optical technology have been utilized to provide excellent instrument reliability and to assure highly accurate results. High throughput optics combined with a modern electronic design provide excellent sensitivity and stability for all manner of sample measurements.

Full Line of Sampling Accessories

A full complement of accessories is available for the V-700 series of UV-Visible-NIR spectrophotometers for virtually any biological, material science or routine QA/QC measurement. Some accessories are common across the V-700 Series and others are instrument specific. Refer to the instrument brochure for accessories specific to your desired instrument.

Options include a wide variety of liquid cell holders, micro and ultra-micro cell holders, flow cell units and accessories for solid samples. Advanced accessories, include automated cell changers, sippers, integrating spheres, automated and manual absolute reflectance accessories and programmable temperature control systems are available for any of the V-700 instrument systems.


Optical System  

Czerny-Turner mount
Single monochromator
Fully symmetrical double beam type

Light source   Halogen lamp, Deuterium lamp
Wavelength range   190 to 900 nm
Wavelength accuracy   +/-0.2 nm (at 656.1 nm)
Wavelength repeatability   +/-0.5 nm
Spectral bandwidth (SBW)   0.1, 0.2, 0.5, 1, 2, 5, 10 nm
L2, L5, L10 nm (low stray light mode)
M1, M2 nm (micro cell mode)
Stray light   1 % (198 nm KCL 12 g/L aqueous solution)
0.005 % (220 nm NaI 10 g/L aqueous solution)
0.005 % (340 nm NaNO2 50 g/L aqueous solution)
0.005 % (370 nm NaNO2 50 g/L aqueous solution)
SBW: L2 nm
Photometric range   -4~4 Abs
Photometric accuracy   +/-0.0015 Abs (0 to 0.5 Abs)
+/-0.0025 Abs (0.5 to 1 Abs)
+/-0.3 %T
Tested with NIST SRM 930D
Photometric repeatability   +/-0.0005 Abs (0 to 0.5 Abs)
+/-0.0005 Abs (0.5 to 1 Abs)
Tested with NIST SRM 930D
Scanning speed   10~4000 nm/min (8000 nm/min in preview mode)
Slew speed   12,000 nm/min
RMS noise   0.00003 Abs
(0 Abs, wavelength: 500 nm, measurement time: 60 sec, SBW: 2 nm)
Baseline stability   0.0003 Abs/hour
(Value obtained more than one hour after turning on the source,
when the room temperature is stabilized, wavelength: 250 nm,
response: slow nd SBW: 2 nm)
Baseline flatness   +/-0.0002 Abs (200 - 850 nm)
Detector   Photomultiplier tube
Dimensions and weight   460(W) x 602(D) x 268(H) mm, 27 kg
Power requirements   150 VA
Installation requirements   Room temperature: 15-30 Celsius, humidity: below 85%


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